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bioimaging approach combining fast fourier transform (fft) with gabor filtering  (Innovative Bioimaging)

 
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    Innovative Bioimaging bioimaging approach combining fast fourier transform (fft) with gabor filtering
    Analysis <t>of</t> <t>collagen</t> organization in ovarian tumor sections of wild-type and lumican-deficient mice. ( a , b ) Representative microphotographs of s.c. allograft sections stained with HES (top panel, original magnification 20×, scale bar 500 µm) in Lum +/+ ( a ) and Lum −/− mice ( b ); ( c – f ) Picrosirius red and SHG image analyses of ovarian allografts in tumors implanted in Lum +/+ mice ( c , d ) and in tumors from Lum −/− mice ( e , f ); ( c , e ) Collagen SHG images from ID8 ovarian tumors (original magnification 20×); ( d , f ) Ovarian tumor sections stained with Picrosirius red and viewed under widefield cross-polar optics (original magnification 20×, scale bar 50 µm). Birefringence of collagen fibers allows distinction between type I (red) and type III (green) collagens; ( g ) Analysis of collagen fibers intensity by SHG in tumors and healthy tissues present in each section (mean ± SD, ns: not significant); ( h ) Analysis of tumor ECM collagen organization from images derived from <t>Gabor</t> filtering and FFT, processed on Picrosirius red images (mean ± SD, ns: not significant); ( i ) Quantification on Picrosirius red stained sections of the relative distribution of red pixels (corresponding to type I collagen) in tumor ECM of Lum +/+ and Lum −/− sections (mean ± SD, * p < 0.05); ( j ) Quantification on Picrosirius red stained sections of the relative distribution of green pixels (corresponding to type III collagen) within tumors of Lum +/+ and Lum −/− sections (mean ± SD, * p < 0.05).
    Bioimaging Approach Combining Fast Fourier Transform (Fft) With Gabor Filtering, supplied by Innovative Bioimaging, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bioimaging+approach+combining+fast+fourier+transform+(fft)+with+gabor+filtering/bioimaging+approach+combining+fast+fourier+transform++fft++with+gabor+filtering/pmc08656468-108-17-8
    Average 90 stars, based on 1 article reviews
    bioimaging approach combining fast fourier transform (fft) with gabor filtering - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Assessment of Ovarian Tumor Growth in Wild-Type and Lumican-Deficient Mice: Insights Using Infrared Spectral Imaging, Histopathology, and Immunohistochemistry"

    Article Title: Assessment of Ovarian Tumor Growth in Wild-Type and Lumican-Deficient Mice: Insights Using Infrared Spectral Imaging, Histopathology, and Immunohistochemistry

    Journal: Cancers

    doi: 10.3390/cancers13235950

    Analysis of collagen organization in ovarian tumor sections of wild-type and lumican-deficient mice. ( a , b ) Representative microphotographs of s.c. allograft sections stained with HES (top panel, original magnification 20×, scale bar 500 µm) in Lum +/+ ( a ) and Lum −/− mice ( b ); ( c – f ) Picrosirius red and SHG image analyses of ovarian allografts in tumors implanted in Lum +/+ mice ( c , d ) and in tumors from Lum −/− mice ( e , f ); ( c , e ) Collagen SHG images from ID8 ovarian tumors (original magnification 20×); ( d , f ) Ovarian tumor sections stained with Picrosirius red and viewed under widefield cross-polar optics (original magnification 20×, scale bar 50 µm). Birefringence of collagen fibers allows distinction between type I (red) and type III (green) collagens; ( g ) Analysis of collagen fibers intensity by SHG in tumors and healthy tissues present in each section (mean ± SD, ns: not significant); ( h ) Analysis of tumor ECM collagen organization from images derived from Gabor filtering and FFT, processed on Picrosirius red images (mean ± SD, ns: not significant); ( i ) Quantification on Picrosirius red stained sections of the relative distribution of red pixels (corresponding to type I collagen) in tumor ECM of Lum +/+ and Lum −/− sections (mean ± SD, * p < 0.05); ( j ) Quantification on Picrosirius red stained sections of the relative distribution of green pixels (corresponding to type III collagen) within tumors of Lum +/+ and Lum −/− sections (mean ± SD, * p < 0.05).
    Figure Legend Snippet: Analysis of collagen organization in ovarian tumor sections of wild-type and lumican-deficient mice. ( a , b ) Representative microphotographs of s.c. allograft sections stained with HES (top panel, original magnification 20×, scale bar 500 µm) in Lum +/+ ( a ) and Lum −/− mice ( b ); ( c – f ) Picrosirius red and SHG image analyses of ovarian allografts in tumors implanted in Lum +/+ mice ( c , d ) and in tumors from Lum −/− mice ( e , f ); ( c , e ) Collagen SHG images from ID8 ovarian tumors (original magnification 20×); ( d , f ) Ovarian tumor sections stained with Picrosirius red and viewed under widefield cross-polar optics (original magnification 20×, scale bar 50 µm). Birefringence of collagen fibers allows distinction between type I (red) and type III (green) collagens; ( g ) Analysis of collagen fibers intensity by SHG in tumors and healthy tissues present in each section (mean ± SD, ns: not significant); ( h ) Analysis of tumor ECM collagen organization from images derived from Gabor filtering and FFT, processed on Picrosirius red images (mean ± SD, ns: not significant); ( i ) Quantification on Picrosirius red stained sections of the relative distribution of red pixels (corresponding to type I collagen) in tumor ECM of Lum +/+ and Lum −/− sections (mean ± SD, * p < 0.05); ( j ) Quantification on Picrosirius red stained sections of the relative distribution of green pixels (corresponding to type III collagen) within tumors of Lum +/+ and Lum −/− sections (mean ± SD, * p < 0.05).

    Techniques Used: Staining, Derivative Assay



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    Innovative Bioimaging bioimaging approach combining fast fourier transform (fft) with gabor filtering
    Analysis <t>of</t> <t>collagen</t> organization in ovarian tumor sections of wild-type and lumican-deficient mice. ( a , b ) Representative microphotographs of s.c. allograft sections stained with HES (top panel, original magnification 20×, scale bar 500 µm) in Lum +/+ ( a ) and Lum −/− mice ( b ); ( c – f ) Picrosirius red and SHG image analyses of ovarian allografts in tumors implanted in Lum +/+ mice ( c , d ) and in tumors from Lum −/− mice ( e , f ); ( c , e ) Collagen SHG images from ID8 ovarian tumors (original magnification 20×); ( d , f ) Ovarian tumor sections stained with Picrosirius red and viewed under widefield cross-polar optics (original magnification 20×, scale bar 50 µm). Birefringence of collagen fibers allows distinction between type I (red) and type III (green) collagens; ( g ) Analysis of collagen fibers intensity by SHG in tumors and healthy tissues present in each section (mean ± SD, ns: not significant); ( h ) Analysis of tumor ECM collagen organization from images derived from <t>Gabor</t> filtering and FFT, processed on Picrosirius red images (mean ± SD, ns: not significant); ( i ) Quantification on Picrosirius red stained sections of the relative distribution of red pixels (corresponding to type I collagen) in tumor ECM of Lum +/+ and Lum −/− sections (mean ± SD, * p < 0.05); ( j ) Quantification on Picrosirius red stained sections of the relative distribution of green pixels (corresponding to type III collagen) within tumors of Lum +/+ and Lum −/− sections (mean ± SD, * p < 0.05).
    Bioimaging Approach Combining Fast Fourier Transform (Fft) With Gabor Filtering, supplied by Innovative Bioimaging, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bioimaging+approach+combining+fast+fourier+transform+(fft)+with+gabor+filtering/bioimaging+approach+combining+fast+fourier+transform++fft++with+gabor+filtering/pmc08656468-108-17-8
    Average 90 stars, based on 1 article reviews
    bioimaging approach combining fast fourier transform (fft) with gabor filtering - by Bioz Stars, 2026-11
    90/100 stars
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    Analysis of collagen organization in ovarian tumor sections of wild-type and lumican-deficient mice. ( a , b ) Representative microphotographs of s.c. allograft sections stained with HES (top panel, original magnification 20×, scale bar 500 µm) in Lum +/+ ( a ) and Lum −/− mice ( b ); ( c – f ) Picrosirius red and SHG image analyses of ovarian allografts in tumors implanted in Lum +/+ mice ( c , d ) and in tumors from Lum −/− mice ( e , f ); ( c , e ) Collagen SHG images from ID8 ovarian tumors (original magnification 20×); ( d , f ) Ovarian tumor sections stained with Picrosirius red and viewed under widefield cross-polar optics (original magnification 20×, scale bar 50 µm). Birefringence of collagen fibers allows distinction between type I (red) and type III (green) collagens; ( g ) Analysis of collagen fibers intensity by SHG in tumors and healthy tissues present in each section (mean ± SD, ns: not significant); ( h ) Analysis of tumor ECM collagen organization from images derived from Gabor filtering and FFT, processed on Picrosirius red images (mean ± SD, ns: not significant); ( i ) Quantification on Picrosirius red stained sections of the relative distribution of red pixels (corresponding to type I collagen) in tumor ECM of Lum +/+ and Lum −/− sections (mean ± SD, * p < 0.05); ( j ) Quantification on Picrosirius red stained sections of the relative distribution of green pixels (corresponding to type III collagen) within tumors of Lum +/+ and Lum −/− sections (mean ± SD, * p < 0.05).

    Journal: Cancers

    Article Title: Assessment of Ovarian Tumor Growth in Wild-Type and Lumican-Deficient Mice: Insights Using Infrared Spectral Imaging, Histopathology, and Immunohistochemistry

    doi: 10.3390/cancers13235950

    Figure Lengend Snippet: Analysis of collagen organization in ovarian tumor sections of wild-type and lumican-deficient mice. ( a , b ) Representative microphotographs of s.c. allograft sections stained with HES (top panel, original magnification 20×, scale bar 500 µm) in Lum +/+ ( a ) and Lum −/− mice ( b ); ( c – f ) Picrosirius red and SHG image analyses of ovarian allografts in tumors implanted in Lum +/+ mice ( c , d ) and in tumors from Lum −/− mice ( e , f ); ( c , e ) Collagen SHG images from ID8 ovarian tumors (original magnification 20×); ( d , f ) Ovarian tumor sections stained with Picrosirius red and viewed under widefield cross-polar optics (original magnification 20×, scale bar 50 µm). Birefringence of collagen fibers allows distinction between type I (red) and type III (green) collagens; ( g ) Analysis of collagen fibers intensity by SHG in tumors and healthy tissues present in each section (mean ± SD, ns: not significant); ( h ) Analysis of tumor ECM collagen organization from images derived from Gabor filtering and FFT, processed on Picrosirius red images (mean ± SD, ns: not significant); ( i ) Quantification on Picrosirius red stained sections of the relative distribution of red pixels (corresponding to type I collagen) in tumor ECM of Lum +/+ and Lum −/− sections (mean ± SD, * p < 0.05); ( j ) Quantification on Picrosirius red stained sections of the relative distribution of green pixels (corresponding to type III collagen) within tumors of Lum +/+ and Lum −/− sections (mean ± SD, * p < 0.05).

    Article Snippet: To assess the basketweave structure of collagen, an innovative bioimaging approach combining Fast Fourier Transform (FFT) with Gabor filtering was applied [ ].

    Techniques: Staining, Derivative Assay